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Duke CareersPosted 10/19/2026full-timeDurham • North Carolina • United StatesJuniorMid-Level💰 $52,477 - $79,504 per yearWebsite
Core Competencies
Role fitCore Competencies
Use this summary to align your resume positioning with the role.
Demonstrates expertise in molecular biology techniques, including PCR, cloning, and flow cytometry, while maintaining regulatory-quality documentation and managing laboratory operations. Proficient in generating and characterizing cell lines for cancer immunotherapy applications.
Highest-signal resume keywords
Molecular CloningFlow CytometryLentiviral TransductionCell Line AuthenticationCancer Biology
About the role
Key responsibilities & impact- Generate molecular and cellular reagents for the cancer immunotherapy program
- Design, construct and validate mammalian expression and lentiviral vectors encoding ESR1-mutant and HER2 sequences, including fluorescent protein fusions
- Perform primer design, PCR, cloning, mutagenesis, bacterial transformation, plasmid preparation and sequence verification
- Maintain organized repositories of plasmids, sequences, maps and glycerol stocks
- Engineer breast and other tumor cell lines through transfection and lentiviral transduction
- Select, expand and clonally isolate stable antigen-expressing cell lines
- Characterize transgene expression, localization and stability using flow cytometry, immunoblot, qRT-PCR and fluorescence microscopy
- Maintain cell banks, cryopreservation, mycoplasma testing, authentication and records
- Perform and optimize cell motility and invasion assays
- Conduct live-cell, time-lapse and confocal fluorescence imaging; process images and quantify motility parameters
- Prepare target cells and reagents for immune function assays
- Perform T-cell co-culture, proliferation, cytotoxicity, flow cytometry, ELISpot, ELISA and intracellular cytokine assays
- Isolate and handle human peripheral blood mononuclear cells
- Operate and maintain technical laboratory instrumentation and analysis software
- Supply constructs, cell lines and reagents for therapeutic cancer vaccine and preclinical immunogenicity studies
- Assist with in vitro and in vivo study readouts, including tissue and specimen preparation
- Collect, evaluate and interpret experimental data; prepare reports, analyses, figures and recommendations
- Maintain electronic laboratory notebooks, written procedures and regulatory-quality documentation
- Confer with the principal investigator on experiments, protocols and interpretation of results
- Review scientific literature to support experimental design and procedure development
- Supervise, train and instruct residents, interns, students and lower-level personnel
- Order and manage reagents and supplies; maintain equipment and records
- Comply with institutional safety and approved biosafety protocols
Requirements
What you’ll need- Bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field
- Two years of research experience
- A related master's degree may offset required years of experience on a 1:1 basis
- Equivalent combination of relevant education and/or experience
- Preferred: doctoral or master's level training in cancer biology, molecular biology, immunology or a related field
- Preferred: direct experience with ER/HER2 breast cancer model systems, oncogenic isoform biology or tumor antigen expression systems
- Preferred: experience generating fluorescent protein reporter lines and applying them to motility or immune killing assays
- Preferred: familiarity with preclinical study documentation supporting regulatory submissions
- Ability to work onsite
- Experience with molecular cloning, PCR, restriction and seamless cloning, site-directed mutagenesis, bacterial transformation, plasmid preparation and sequence verification
- Experience with transfection, lentiviral transduction, flow cytometry, immunoblot, qRT-PCR and fluorescence microscopy
- Experience with cell cryopreservation, mycoplasma testing and cell line authentication
- Experience with scratch/wound-healing, transwell migration and invasion, and matrix-based assays
- Experience with live-cell, time-lapse and confocal fluorescence imaging, image processing, tracking and quantitative motility analysis
- Experience with antigen-specific T cell co-culture, proliferation and cytotoxicity assays
- Experience with multiparameter flow cytometry, ELISpot, ELISA, intracellular cytokine staining and human peripheral blood mononuclear cells
- Ability to operate and maintain flow cytometers, imaging systems, plate readers and associated analysis software
- Ability to maintain regulatory-quality laboratory documentation
- Ability to train laboratory personnel
- Ability to manage reagents, supplies, equipment and records
- Compliance with institutional biosafety, chemical safety and occupational health requirements
Benefits
Comp & perks- Health insurance plans
- Generous paid time off
- Retirement programs with employer contributions
- Tuition assistance for employees and their children
- Comprehensive medical and dental care programs
- Family-friendly and cultural programs
ATS Keywords
✓ Tailor your resumeApplicant Tracking System Keywords
Tip: use these terms in your resume and cover letter to boost ATS matches.
Hard Skills
PCRCloningMutagenesisTransfectionQRT-PCRImmunoblotFluorescence MicroscopyCell CryopreservationELISpotELISA
Soft Skills
TrainingSupervisionCollaboration
Tools & Technologies
Flow CytometersImaging SystemsPlate ReadersAnalysis Software
Industry Keywords
Cancer ImmunotherapyTumor Antigen ExpressionPreclinical StudiesBiosafety ProtocolsRegulatory Documentation